2014 Toyota Prius 1st Tune Up

http://www.toyota.com/t3Portal/document/omms-s/T-MMS-14Prius/pdf/2014_Toyota_Prius_WMG_lr.pdf
Explains the Toyota service intervals (there really is no tune up in the way there was in the 1980s)
At 60,000 miles/72 months (page 46) you should have had several air filters replaced.
At 100,000 miles/120 months (page 51) you should replace several coolants.
At 120,000 miles 144 months (page 54) you should replace the spark plugs

Outside of Toyota's service schedule; I would replace the ATF WS in the transaxle every 60,000 miles.

 

What do you mean by "tune up"? I don't think it really exists anymore, in the traditional sense, and never was all that definitive.

I would just follow the US Warranty and Maintenance Booklet, except for additional transaxle fluid drain-and-fill, say at 30K miles, then every 60K miles thereafter (or sooner for the first, imho). Also, note: there are two levels of brake inspection: the cursory "visual" inspection at tire rotation, and a more thorough inspection involving pulling the caliper off. The latter is every 30K miles or 36 months.